Pramod K. Gupta,Diane G. Holmstrom,Pamela L. Carlson
申请号:
US13602983
公开号:
US08999713B2
申请日:
2012.09.04
申请国别(地区):
US
年份:
2015
代理人:
摘要:
The invention provides methods for multiplying conifer embryogenic tissue. The methods of the invention comprise the steps of: (a) culturing conifer embryogenic tissue in or on a solid multiplication medium comprising one or more growth hormones and (b) culturing the conifer embryogenic tissue multiplied in step (a) in or on a liquid multiplication medium comprising one or more growth hormones, wherein the initial concentration of growth hormones in the solid multiplication medium of step (a) is greater than the initial concentration of growth hormones in the liquid multiplication medium of step (b).