BRAHMANDAM, ARCHANA,THOMPSON, LUCAS JAMES,MORTENSEN, DEBORAH,FILVAROFF, ELLEN
申请号：
CA3080509
公开号：
CA3080509A1
申请日:
2018.11.01
申请国别(地区):
CA
年份:
2019
代理人:
摘要：
Provided herein are methods for producing engineered T cells that express a recombinant receptor, such as for use in cell therapy. In some aspects, the provided methods include one or more steps for incubating the cells under stimulating conditions, introducing a recombinant polypeptide to the cells through transduction or transfection, and/or cultivating the cells under conditions that promote proliferation and/or expansion, in which one or more steps is carried out in the presence of an agent that inhibits mammalian target of rapamycin (mTOR) activity. In some aspects, cultivation is performed in the presence of an agent that inhibits mammalian target of rapamycin (mTOR) activity. In some aspects, the provided methods produce genetically engineered T cells with improved persistence and/or anti-tumor activity in vivo.
