The invention pertains to methods of improving the yield and preserving the quality of recombinantly produced proteins and polypeptides in a production bioreactor process by manipulating or controlling the temperature of a bioreactor process that precedes the ultimate production bioreactor process. In particular embodiments the invention provides such methods as well as recombinant proteins and polypeptides produced using such methods, formulations thereof, and methods of treating patients using such proteins, polypeptides, and formulations.
