PROBLEM TO BE SOLVED: To provide a sterilization method with which an effective sterilizing effect can be acquired for heat-resistant bacteria spores with a more moderate condition compared to conventional cases and with which influence upon the quality of sterilization subjects is insignificant.SOLUTION: The sterilization method includes: a first heating treatment step which is mainly executed to give damages to spores； and, thereafter, a second heating treatment step which applies a treatment at a lower temperature than that of the first heating treatment step to mainly execute inactivation of the damaged spores.SELECTED DRAWING: Figure 1COPYRIGHT: (C)2018,JPO&INPIT【課題】 耐熱性の細菌胞子に対して、従来と比べて温和な加熱条件で、効果的な殺菌効果が得られ、かつ、被殺菌物の品質への影響が小さい殺菌方法を提供することを課題とする。【解決手段】 胞子に対して損傷を付与することを主目的とする第１の加熱処理工程と、その後に、損傷を受けた胞子を不活性化することを主目的とする、第１の加熱処理工程より低い温度で処理する第２の加熱処理工程、を備える。【選択図】図１
