A method of culturing a three-dimensional tissue including living organism configuration in which tissue culturing can be performed in the state and configuration approximate to those of tissue within living organism. There is provided a method of culturing a three-dimensional tissue including living organism configuration, comprising the steps of: (a1) obtaining by photographing the image of diseased site or peripheral tissue of transplant planned (b1) preparing three-dimensional living organism configuration data of the site from the image information on the photographed diseased site or peripheral tissue of transplant planned (c1) preparing a mold on the basis of the three-dimensional living organism configuration data (d1) preparing a scaffold with three-dimensional living organism configuration by the use of the mold (e1) preparing a tissue with three-dimensional living organism configuration by introduction of cells on the scaffold and (f1) culturing the tissue.
