A sterile bag for containing non-sterile objects includes: a body； a sealed first end； a second end forming a cuff and an opening to an internal portion of the bag when in a first position； a visible demarcation located circumferentially along the cuff dividing the bag into two sides； a closure mechanism located along the cuff on the proximal side of the visible demarcation such that when the second end is in a second position there is no cuff and the closure mechanism can be used to seal the bag with the visible demarcation sealed within the bag. In use, a first user with scrubbed hands may touch the bag only on one side of the visible demarcation and a second user with non-scrubbed hands may touch the bag only on the other side of the visible demarcation.
