The present invention analyzes removing efficiency of haemoglobin from the porcine lung tissue in the general characteristic, histochemical properties, chemical composition, compares different solvents and also establishes the optimum extraction conditions of telopeptide-poor collagen from porcine lung. The present invention further obtains the maximal yield of telopeptide-poor collage from porcine lung (TPCPL) by pepsin solubilisation for 72 hours. The results of SDS-PAGE and FTIR spectrum analysis show that the TPCPL sample is free of protein contaminant and denatured product and has the type I collagen as a major component.本發明分析豬肺臟之一般特性、組織化學特性、化學組成及比較不同溶劑對移除豬肺組織中血紅素效率之影響,並建立豬肺臟中低終端胜肽膠原蛋白之最佳化萃取模式。本發明進一步以胃蛋白酶萃取72小時可得最高產率之低終端胜肽膠原蛋白,藉電泳鑑定並無非膠原蛋白蛋白質之污染,且於傅立葉轉換紅外線光譜(FTIR)光譜發現無變性產物存在,並以第一型膠原蛋白為主要組成。10‧‧‧哺乳類動物肺臟前處理11‧‧‧去除肺臟之血紅蛋白12‧‧‧酵素反應處理13‧‧‧鹽析反應14‧‧‧冷凍乾燥
