An isolated nucleic acid that contains a nucleotide sequence that encodes the polypeptide of SEQ ID NO: 3. The polypeptide of SEQ ID NO: 3 specifically binds to stage-specific embryonic antigen 4 (SSEA4). Also disclosed is a recombinant cell comprising the isolated nucleic acid described above, a viral vector containing the above isolated nucleic acid, and an isolated polypeptide including the sequence of SEQ ID NO: 3. Provided as well is a chimeric antigen receptor (CAR) that includes a single chain Fv having the sequence of SEQ ID NO: 3 and specifically binding to SSEA4. Moreover, a method is disclosed for treating a tumor by transducingin vitrothe T cells of a subject having a tumor expressing SSEA4with a vector that encodes the CAR, expanding the transduced T cells, and infusing the expanded transduced T cells into the subject, whereby an anti-tumor T cell response is raised.一種經分離的核酸，其包含一編碼序列辨識編號：3的多肽之核苷酸序列。序列辨識編號：3的多肽專一性結合至階段特異性胚胎抗原4(SSEA4)。亦揭示的是一種包含上述經分離的核酸之重組型細胞、一種包含上面經分離的核酸之病毒載體，以及一種包含序列辨識編號：3的序列之經分離的多肽。亦提供的是一種嵌合抗原受體(CAR)，其包含一具有序列辨識編號：3的序列並且專一性結合至SSEA4之單鏈Fv。此外，揭示一種用於治療一腫瘤之方法，其是藉由：於活體外使用一編碼CAR的載體來轉導一具有一表現SSEA4的腫瘤之個體的T細胞，擴增該經轉導的T細胞，以及將所擴增之經轉導的T細胞注入該個體中，藉此抗腫瘤的T細胞反應被提升。
