Agricultural Genomics Institute, Chinese Academy of Agricultural Sciences;Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences
发明人:
申请号：
EP19784808.8
公开号：
EP3719132A1
申请日:
2019.04.04
申请国别(地区):
EP
年份:
2020
代理人:
摘要：
Disclosed is a method for overcoming self-incompatibility of diploid potatoes, which comprises: (1) selecting a target fragment； (2) constructing a gene-targeting recombinant vector； (3) achieving a loss-of-function mutation of the intracellular S-RNase gene； (4) regenerating a plurality of potato plants； (5) specifically amplifying a DNA segment of the S-RNase gene in a regenerated plant, wherein the DNA segment containing the target fragment； (6) selecting a regenerated plant in which the S-RNase gene is edited； (7) further screening the selected gene-edited plant for a diploid gene-edited plant line； (8) propagating and planting the selected gene-edited plant line, and identifying the self-compatible phenotype at the flowering stage； and (9) sequencing the gene amplification products of the harvested offspring of the self-compatible plant, and detecting the inheritance and isolation of the offspring in which the target gene is edited. The advantage of the present invention lies in that a simple, accurate and efficient method for overcoming the self-incompatibility of diploid potatoes is found.
