The present invention relates to a process of extracting an oil phase from wet biomass of micro-organisms via a biphasic extract containing an aqueous phase and an oil phase, the process comprising: €¢ combining the wet biomass with a solvent containing at least 60 wt.% of C 1-5 monoalcohol, said biomass containing 10-95 wt.% of water and 5-85% by weight of dry matter of lipids selected from triglycerides, diglycerides, monoglycerides, phosphatides, free fatty acids and combinations thereof； €¢ isolating a liquid phase from the combination of biomass and solvent, said liquid phase containing 5-85 wt.% of C 1-5 monoalcohol, 10-85 wt.% of water and at least 5 wt.% of dissolved and/or dispersed components originating from the biomass, including at least 5% of lipids by weight of C 1-5 monoalcohol； €¢ reducing the concentration of C 1-5 monoalcohol in the liquid phase to less than 10 wt.% whilst maintaining a water content of at least 15 wt.% to produce a biphasic extract containing an aqueous phase and an oil phase； and €¢ separating the oil phase from the aqueous phase. The present process offers the advantage that wet biomass is used as a starting material, which means that drying steps can be avoided. Furthermore, this process does not require the use of apolar organic solvents. The process produces É3-PUFA containing oil in high yield.
