A composition for preventing and treating arthritis includes a DNA fragment mixture and a matrix metalloproteinase (MMP) production inhibitor capable of overcoming the problem of MMP production induced when using the DNA fragment mixture in the prevention and treatment of arthritis, thereby being able to inhibit inflammatory pain and to make cartilage regeneration possible. The composition may inhibit matrix metalloproteinase production induced by the DNA fragment mixture, and, thus, may be effectively used without adverse effects for induction of chondrocyte proliferation, repair of damaged chondrocytes, regeneration of cartilage, and relief of arthritis.
