The present invention is a method for culturing cells in which a seed medium containing cells is added to an initial medium, and cell culture is started in the initial medium, wherein an additive for a biological medium is added to the initial medium, And a method for culturing cells, characterized in that the amount of the C-terminal amide of the produced protein is controlled by the copper content at the start of cell culture in the initial medium.本発明は、細胞を含む種培地を初発培地に添加し、初発培地中で細胞の培養を開始する細胞の培養方法であって、初発培地に生物由来培地用添加物が添加されていること、および、当該初発培地の細胞培養開始時の銅含量により産生タンパク質のC末端アミド体の量を制御することを特徴とする、細胞の培養方法である。
