The present invention relates to a method for separating one or more oligosaccharide compound(s) from an oligosaccharide containing mixture, the method comprises the steps of (i) providing the oligosaccharide containing mixture； (ii) contacting the oligosaccharide containing mixture with a chromatographic support allowing one or more oligosaccharide compound(s) present in the oligosaccharide containing mixture to be retained by the chromatographic support； (iii) obtaining a unretained, flow through fraction from the chromatographic support comprising a protein, a cell, a cell debris, a nucleic acid and/or an enzyme； (iv) optionally washing the chromatographic support； (v) subjecting the chromatographic support to at least one elution buffer obtaining one or moreoligosaccharide compound(s) from the chromatographic support； and wherein the chromatographic support comprises an adsorbent comprising one or more ligand capable of binding the one or more oligosaccharide compound(s) from the oligosaccharide containing mixture and wherein the one or more ligand comprise a serotonin compound, or a derivative thereof, wherein the oligosaccharide containing mixture is a preliminary diary source or a whey material, and wherein the chromatographic support is a column chromatography support, wherein the column chromatography support is an Expanded Bed Chromatography.
