Richard R. Koepsel;Hironobu Murata;Chad Cummings;Alan J. Russell
发明人:
Alan J. Russell,Richard R. Koepsel,Chad Cummings,Hironobu Murata
申请号：
US14785868
公开号：
US20160101190A1
申请日:
2014.04.22
申请国别(地区):
US
年份:
2016
代理人:
摘要：
Using a novel water-soluble, active ester amide-containing functionalized controlled radical polymerization initiator, stimuli responsive polymers have been grown from the surface of a protein, exemplified by chymotrypsin or any protein having surface amino acids that will covalently bind to the active ester amide-containing functionalized initiator. It is shown that changes in temperature or pH can change the conformation of the polymer surrounding the enzyme, which in turn enabled the rational tailoring of enzyme activity and stability. This method has afforded an increase in the activity and stability of the enzyme by an order of magnitude at pH's where the enzyme is usually inactive or unstable. Multimodal temperature responsive protein-block copolymer conjugates are described.
