PROBLEM TO BE SOLVED: To provide a manufacturing method of a microneedle pharmaceutical preparation which is capable of stably forming a needle part by using a liquid composition in which a pharmaceutical preparation is dissolved into aqueous solvent.SOLUTION: A manufacturing method of a microneedle pharmaceutical preparation 1 comprises an adhesion part 10 including a sheet-like base material 12 and a plurality of needle parts 14 protruded from one side of the base material 12, in which a liquid composition X, in which a pharmaceutical preparation is dissolved into aqueous solvent, is charged into a mold 100 having temperature Twhich satisfies the condition of 50(°C)≤T, and dried under an environment having temperature Twhich satisfies the condition of T
