A lens holder for holding a vitrectomy lens during a surgical procedure may include a support ring sized and shaped to receive a portion of the vitrectomy lens to provide visualization of a surgical site in an eye of the patient. The support ring may be configured to rest upon the eye of the patient during a surgical treatment procedure performed on the eye. The lens holder may also include a transparent, flexible membrane disposed along a bottom plane of the support ring and configured to conform to a surface feature on the eye. It may also include an overflow trough to control contact of a wetting agent with the eye.