The present invention relates to a method of positioning a complete immunoglobulin form of an antibody in the cytoplasm via active cell penetration. The present invention also relates to a light chain variable region (VL) that induces an intact immunoglobulin form of an antibody to actively penetrate the cell membrane of a living cell and to be located in the cytoplasm, and an antibody comprising the same About. The present invention also relates to a bioactive molecule selected from the group consisting of peptides, proteins, small molecule drugs, nanoparticles and liposomes fused to the antibody. The present invention also provides a composition for preventing, treating or diagnosing cancer comprising the antibody or a bioactive molecule selected from the group consisting of peptides, proteins, small molecule drugs, nanoparticles and liposomes fused thereto. Related to things. The present invention also relates to the polynucleotide encoding the light chain variable region and the antibody. The present invention also relates to a method for producing an antibody that penetrates into the cell and is located in the cytoplasm. According to the method of positioning the complete immunoglobulin form of the antibody provided in the present invention in the cytoplasm through active cell penetration, the antibody is allowed to penetrate inside living cells without a special external protein transmission system. Can be distributed in the cytoplasm. Further, using the light chain variable region having cytoplasmic penetrating ability according to the present invention and the antibody in the form of a complete immunoglobulin containing the same, high antigen specificity and high affinity of the human antibody heavy chain variable region (VH) It can be added to the inside of the cell without affecting the cell, and the property of remaining in the cytoplasm can be imparted, and through this, it exists in the cytoplasm classified as a target substance for disease treatment us
