PROBLEM TO BE SOLVED: To provide a technique of producing more homogeneous differentiated cell populations from pluripotent stem cells (pSP) derived from human.SOLUTION: Disclosed is a cell population in which differentiation ability of neural progenitor cells, differentiated neurons, glial cells, and astrocytes is determined. The population is obtained by culturing a stem cell population (embryonic stem cells and the like) in a cocktail of growth conditions that initiate differentiation of pPs cells in a culture medium containing at least two ligands that bind with growth factor receptors selected from EGF, bFGF, PDGF, IGF-1, and antibodies to the receptors of these ligands, and that establish neural progenitor populations. The progenitors can be further differentiated in culture into a variety of different neural phenotypes, including dopaminergic neurons. A method of producing the differentiated cell population or the neuronal progenitors in large quantities for use in drug screening and the treatment of neurological disorders is also disclosed.SELECTED DRAWING: None【課題】ヒト由来の多能性（ｐＳＰ）細胞から、より均一な分化細胞集団を生じさせる技術の提供。【解決手段】神経前駆細胞、分化したニューロン、グリア細胞、及びアストロサイトの分化能が決定される細胞集団。本集団は、幹細胞集団（胚性幹細胞など）を、ＥＧＦ、ｂＦＧＦ、ＰＤＧＦ、ＩＧＦ－１及びこれらのリガンドの受容体に対する抗体から選択される、成長因子受容体と結合する少なくとも２つのリガンドを含む培地中でｐＰＳ細胞を分化を惹起し、神経前駆細胞集団を確立する成長条件の混液中で培養することによって得られ、前駆細胞を培養下で、ドーパミン作動性ニューロンを含むさまざまな異なる神経表現型へとさらに分化でき、分化細胞集団または神経前駆細胞を、薬物スクリーニングおよび神経疾患の治療に用いるために大量に作製する方法。【選択図】なし
