PROBLEM TO BE SOLVED: To provide a method for producing a large amount of hepatic progenitor cells from human iPS cells.SOLUTION: A differentiation inducing method comprises introducing, into human induced pluripotent stem cells, a combination of genes of transcription factors which are possessed by fetal and adult hepatic cells, but are not possessed by human induced pluripotent stem cells and adhering the combination of genes to a substrate for monolayer culture in a medium containing a combination of growth-promoting agents for proliferation and differentiation, thereby enabling induction of the differentiation of the human induced pluripotent stem cells to hepatic progenitor cells in a large amount in a short term. A hepatic progenitor cell is produced by the method. A cell composition for transplantation into the liver comprises the cell. The transcription factors include FOXA2, GATA4, HEX and C/EBP&alpha；, and the growth-promoting agents include oncostatin M, an epidermal growth factor, retinoic acid, dexamethasone, insulin, transferrin and a selenite ion.【課題】ヒトｉＰＳ細胞から大量の肝前駆細胞を短期間で製造する方法を開発する。【解決手段】本発明は、胎児や成人の肝細胞が保有し、ヒト人工多能性幹細胞が非保有の転写因子の遺伝子を組合せてヒト人工多能性幹細胞へ導入し、増殖、分化させるための増殖促進剤の組合せを含む培地中で基質に接着して単層培養させることにより、ヒト人工多能性幹細胞から肝前駆細胞へ短期間で大量に分化誘導し得る分化誘導法、該方法で製造された肝前駆細胞、該細胞を含む肝臓への移植用細胞組成物を提供する。前記転写因子としてＦＯＸＡ２、ＧＡＴＡ４、ＨＥＸおよびＣ／ＥＢＰαが、前記増殖促進剤としてオンコスタチンＭ、上皮成長因子、レチノイン酸、デキサメタゾン、インシュリン、トランスフェリンおよび亜セレン酸イオンが挙げられる。【選択図】なし
