LIN, PEI CHENG,林培正,林培正,CHANG, HAN HSIN,张菡馨,張菡馨,CHEN, CHIN CHU,陈劲初,陳勁初,YEH, SHU HSING,叶淑幸,葉淑幸,LEE, LI YA,李丽雅,李麗雅,HSU, JUI HSIA,徐瑞霞,徐瑞霞
申请号：
TW104112814
公开号：
TWI558405B
申请日:
2015.04.22
申请国别(地区):
TW
年份:
2016
代理人:
摘要：
A method of preparing Cordyceps cicadae mycelium active substances for preventing and/or treating xerophthalmia is provided. The method comprises: (a) culturing a Cordyceps cicadae mycelium in a plate media at 15 to 35° C. for 5 to 14 days (b) inoculating the mycelium of step (a) to a flask containing liquid media and culturing it at 15 to 35° C. with a pH of 2 to 8 for 3 days (c) inoculating the mycelium of step (b) to a fermenter tank and culturing it at 15 to 35° C. with a pH of 2 to 8 for 3 days, so as to obtain a Cordyceps cicadae mycelium fermentation liquid (d) freeze-drying and grating the fermentation liquid, so as to obtain a Cordyceps cicadae mycelium powder (e) extracting the powder with solvent, so as to obtain a Cordyceps cicadae mycelium extract and (f) drying the extract, so as to obtained the Cordyceps cicadae mycelium active substances.本發明關於一種用於預防及/或治療乾眼症之蟬花活性物質,係以下列方法製備:(a)取蟬花菌絲體於平板培養基上,於15-35℃培養5-14天;(b)將步驟(a)培養後之蟬花菌絲體接種至燒瓶內,於15-35℃、pH 2-8的條件培養數天;(c)將步驟(b)培養後之蟬花菌絲體接種至一發酵槽內,於15-35℃、pH 2-8、槽壓0.5-1.0kg/cm2且通氣速率0.01-1.5VVM的條件培養3-5天,形成一蟬花菌絲體發酵液;(d)將蟬化菌絲體發酵液冷凍乾燥後磨粉,形成一蟬花菌絲體凍乾粉;(e)將蟬花菌絲體凍乾粉以溶劑萃取,形成蟬花菌絲體萃取液;以及(f)將蟬花菌絲體萃取液乾燥後,獲得蟬花活性物質。本發明之蟬花活性物質亦可用於製備預防及/或治療乾眼症之醫藥組合物。
