A system that can reduce the effect of oxygen inhibition for Nannochloropsis oculata cultivation is disclosed. The cell culture chamber is made of a transparent spinner flask where it contains fluorochemical liquid and Walne’s medium with pH of 8 serving as the oxygen carrier and culture medium, respectively. Both liquids are immiscible and therefore separate into two phases, in which the fluorochemical liquid is on the bottom due to higher density. The spinner flask includes multiple openings. Upon operation, the Walne’s medium must be controlled at 25 ± 2℃, and continuously illuminated using a light source with intensity of 80 μE/m2/s. The magnetic paddle should be set at the interface between Walne’s medium and fluorochemical liquid, and agitated continuously to transfer dissolved oxygen from the culture medium to the fluorinated liquid. The oxygen-carrying fluorochemical liquid will be aspirated to an external gas exchange unit for deoxygenation through nitrogen aeration. Afterward, the deoxygenated fluorochemical liquid is pumped back to the spinner flask for recycling. The fluorochemical liquid in-and-out of the spinner flask goes through different openings, and only one pump is involved in the system.一種可以降低氧氣抑制的擬球藻培養系統。該系統在設置上包括:在一可透光之攪拌槽中置入一氧氣載體以及細胞培養液,其中該氧氣載體為碳氟化合物液體而細胞培養液則為酸鹼值(pH)為8的韋因培養基(Walne’s medium);二者溶液不互溶而分成下、上兩相。該可透光之攪拌槽具有複數個開口。在使用時,韋因培養基的溫度得控制在25±2℃之下,並以照明強度為80 μE/m2/s之單一光源連續照射之;另外該可透光之攪拌槽內的葉片是以連續攪拌的方式混合該氧氣載體以及該培養基。下方之該氧氣載體將可攜帶上方培養液中生物體所產生的氧氣,並由一幫浦自其中一個開口抽離該可透光之攪拌槽而進入一氣體交換槽,並且在該氣體交換槽內與連續注入之氮氣接觸後再由該幫浦抽離,經由另一開口而再次進入該可透光之攪拌槽的下方。20‧‧‧可透光之攪拌槽(密閉三口瓶)60‧‧‧幫浦30‧‧‧換氣瓶50‧‧‧光源10‧‧‧電磁攪拌器40‧‧‧溶氧度計20a、30a‧‧‧全氟碳化物液體20b‧‧‧韋因培養基70、90‧‧‧管線80、95‧‧‧開口1000‧‧‧葉片1500‧‧‧氧氣體積流量計
