A breathing guidance system having an active biofeedback mechanism is provided. The breathing guidance system includes a physiological status detection device, a processing device, a breathing indication device and an instant breathing detection device. The physiological status detection device detects at least one piece of instant physiological status information of the user. The processing device generates an ideal breathing waveform according to the instant physiological status information to further generate a control signal. The breathing indication device generates a breathing indication signal according to the control signal. The instant breathing detection device detects a piece of instant breathing status information of the user. The processing device further generates an instant breathing waveform according to the instant breathing status information to compare the ideal breathing waveform and the instant breathing waveform to generate a breathing condition indication signal.一種具主動生理回饋機制之呼吸導引系統,包含:生理狀態偵測裝置、處理裝置、呼吸提示裝置以及即時呼吸偵測模組。生理狀態偵測裝置偵測使用者之至少一即時生理狀態資訊。處理裝置根據即時生理狀態資訊以及預設呼吸波形產生目標呼吸波形,俾進一步產生控制訊號。呼吸提示裝置根據控制訊號產生呼吸提示訊號。即時呼吸偵測模組偵測使用者之即時呼吸狀態資訊。其中處理裝置更根據即時呼吸狀態資訊以及預設呼吸波形產生即時呼吸波形,並比較目標呼吸波形以及即時呼吸狀態資訊以產生呼吸狀況提示訊號。1‧‧‧呼吸導引系統10‧‧‧生理狀態偵測裝置11‧‧‧即時生理狀態資訊12‧‧‧處理裝置13‧‧‧控制訊號14‧‧‧呼吸提示裝置15‧‧‧呼吸提示訊號16‧‧‧即時呼吸偵測模組17‧‧‧即時呼吸狀態資訊18‧‧‧使用者19‧‧‧呼吸狀況提示訊號