PRODUCTION OF POLIO VIRUS AT HIGH TITERS FOR VACCINE PRODUCTION

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专利权人:

CRUCELL HOLLAND B.V.

发明人:

LEWIS, JOHN, ALFRED

申请号：

NZ59688010

公开号：

NZ596880A

申请日:

2010.07.08

申请国别(地区):

NZ

年份:

2013

代理人:

摘要：

596880 Disclosed is a process for the production of poliovirus, comprising the steps of: a) providing a serum-free suspension culture of cells, which are PER.C6 cells as deposited under ECACC no. 96022940, b) infecting said cells with poliovirus, at a cell density of between 2x106 cells/ml and 150x106 cells/ml, and c) harvesting poliovirus at a time of between 12 and 48 hours after infection.

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