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Bacterial Strain isolated from the genus burkholderia pesticides and metabolites thereof, formulations and uses
专利权人:
MARRONE BIO INNOVATIONS; INC.
发明人:
RATNAKAN ASOLKAR,MARJA KOIVUNEN,PAMELA MARRONE
申请号:
ARP120103151
公开号:
AR087684A1
申请日:
2012.08.24
申请国别(地区):
AR
年份:
2014
代理人:
摘要:
It provide a kind of Burkholderia sp. pathogenicity but without known vertebrates with Pesticide activity (e.g., plants, Algae, arachnids, insects, fungi, nematodes and weeds) and Methods for controlling Algae using these Burkholderia species.Also provided are natural products derived from a crop of this species and the methods to control Algae and / or arachnids using these natural products.Claim 1: a formulation comprising: (a) a Strain isolated or fermentation Broth comprising cells and Culture medium of a Burkholderia cepacia, Burkholderia Burkholderia gladioli plantari not not not, Burkholderia sp. Burkholderia cepacia, Burkholderia multivorans, Burkhalter Olderia sp.It has the following characteristics: (1) Gene sequence of 16S rRNA Sequence which comprises the front has at least 99% identity with the sequence shown in SEQ ID No. 8, 11, 12 and reverse Sequence has at least 99% identity with the sequence shown in seq ID no. 9, 10, 13,14 and 15; (2) Pesticide activity; (3) produces a pesticide Compound selected from the group consisting of: (a) a compound having the following properties: (i) a Molecular Weight of approximately 525 - 555 determined by Liquid Chromatography / mass spectroscopy (LC / MS); (ii) u00b9h r MN values D 6.22, 5.81, 5.69, 5.65 5.66,, 4.64, 4.31, 3.93, 3.21, 3.22, 2.69, 2.62 3.10 3.15,, 2,,26, 2.23, 1.74, 1.15, 1.08, 1.05, 1.02; (iii) has u00b9 u00b3c NMR values 172,99 172,93, D, 169,57, 169,23, 167,59, 130,74, 130,12, 129,93, 128,32, 73,49, 62,95, 59.42, 57,73, 38,39, 38, 35,49, 30,90, 46,40 29,26 18.38 18.59,,, 18.09 17.93 12.51,,,; and (iv) a retention time of the cromatogra Cold High Pressure Liquid (HPLC) of approximately 10 - 15 minutes.In a column c-18 Reverse Phase HPLC using a gradient of acetonitrile: Water (ch u2083cn); (b) a compound having a structure comprising at least oxazolil indole indole residue a residue oxazole, at least, at least one alkyl group substituted carboxylic Ester and at least one group. At least 17 car
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