JARVIUS, Jonas,LIND, Anders,SÖDERSTRÖM, Henrik,GRAWÉ, Jan
申请号：
EPEP2017/059542
公开号：
WO2017/182645A1
申请日:
2017.04.21
申请国别(地区):
WO
年份:
2017
代理人:
摘要：
A method for detecting and characterising a microorganism in a clinical sample includes introducing a clinical sample to a first culture vessel and then simultaneously transporting and incubating the clinical sample in a thermally insulated compartment of a sealable container. A test aliquot is removed from the sample and the remainder of the sample is cultured further. The method further includes removing DNA from said test aliquot and performing nucleic acid tests on said DNA to identify the microorganism and to detect the presence or absence of one or more genetic antimicrobial resistance markers in said microorganism. The nucleic acid tests are performed using: i) one or more nucleic acid probes and/or primers for microbial identification, a said probe or primer being capable of hybridising specifically to, or a said primer being capable of selectively amplifying, a nucleotide sequence which is identificatory of a given microorganism and ii) one or more nucleic acid probes and/or primers for antimicrobial resistance marker detection, a said probe or primer being capable of hybridising specifically to, or a said primer being capable of selectively amplifying, a nucleotide sequence representing a genetic antimicrobial resistance marker. It is detected whether or not said probes and/or primers have hybridised to said DNA and/or said primers have been extended (e.g. an amplification reaction has taken place) and if a microorganism is identified in step then an antimicrobial susceptibility test is performed on said cultured clinical sample.La présente invention concerne un procédé de détection et de caractérisation dun micro-organisme dans un échantillon clinique qui comprend lintroduction dun échantillon clinique dans une première cuve de culture et ensuite, le transport et lincubation simultanés de léchantillon clinique dans un compartiment thermiquement isolé dun récipient scellable. Une aliquote dessai est prélevée à partir de léchantillon et le reste de léchanti
