A biomedical 3D rapid prototyping apparatus includes at least one bio-cartridge, a supply container, a construction platform, an identifying and monitoring device, a disinfection and temperature/humidity regulation device, a casing, and a negative pressure device. The bio-cartridge contains a bio-forming fluid, and has a printhead for ejecting the bio-forming fluid. The supply container stores a bio-building material. The bio-building material is spread in a construction chamber of the construction platform to form a construction layer, and the bio-cartridge is moved to the construction layer to print the bio-forming fluid on the construction layer, so that a cellular tissue is formed. The procedure of spreading the bio-building material and the procedure of printing the bio-forming fluid are repeatedly done, so that a transplantable living tissue or organ is quickly produced.
