KOTHA, Shiva Prasad,WATSON, Andre Ronald,PANDIT, Vaibhav A.
申请号：
ES14846422
公开号：
ES2719112T3
申请日:
2014.09.23
申请国别(地区):
ES
年份:
2019
代理人:
摘要：
An improved nanoparticle for transfecting cells is provided. The nanoparticle includes a core polyplex and a silica coating on the core polyplex and, optionally, a polymer attached to an outer surface of the silica coating , where the polyplex includes an anionic polymer, a cationic polymer, a cationic polypeptide, and a polynucleotide. Also provided is an improved method of modifying intracellular polynucleotides. The method includes contacting a cell with a nanoparticle that includes a core polyplex and a silica coating on the core polyplex and, optionally, a polymer attached to an outer surface of the silica coating, where the polyplex includes an anionic polymer, a cationic polymer, a cationic polypeptide, and a polynucleotide.
