The invention relates to a formulation having biocide and/or phytosanitary properties that is used the form of aerosol, characterized in that said formulation comprises: (a) 20 to 60% by weight of a solution of at least an active biodegradable substance having biocide and/or phytosanitary properties and (b) 40 to 80% by weight of at least a propellant. An aim of the invention is also to use said formulation to disinfect rooms and/or surfaces, as well as to control illnesses from post-harvest vegetables and/or fruits by using the formulation in the form of an aerosol.