RAJ MOHAN BOGU,Venkatarao Gonuguntla,MURTHY KONDRAGUNTA SRI RAMA
申请号：
IN201741008623
公开号：
IN201741008623A
申请日:
2017.03.13
申请国别(地区):
IN
年份:
2018
代理人:
摘要：
The present application relates to a commercially viable process technique for in-vitro production of AMF (Arbuscular Mycorrhizal Fungus) spores. More specifically, the present invention relates to large scale commercially viable process for in-vitro production of AMF spores wherein optimization of large scale production of Mycorrhizal spores (AMF spores) involved a unique split plate method in containers, which is carried out in the following steps: i). Proliferating callus root clumps ex planted in three individual container ii). each container containing 80-11 0 ml of three different media like MW medium, M medium and MSR medium respectively iii). assess the root colonization by Rhizophagus irregu/aris using magnified grid line intersection method iv). number of spores counted and v). whole root length from each container examined to calculate the intra-radical structures of AMF. The AMF spores produced by the process of the present invention are useful in reestablishing the mycorrhizal populations in the soil for uplifting the soil health, plant growth and yield.
