Федеральное государственное бюджетное учреждение науки Тюменский научный центр Сибирского отделения Российской академии наук (RU)
发明人:
Бажин Александр Сергеевич (RU),Бычков Виталий Григорьевич (RU),Нарушко Максим Викторович (RU),Габдуллин Максим Альбертович (RU),Козлов Леонид Борисович (RU),Калёнова Людмила Федоровна (RU)
申请号：
RU2015150608
公开号：
RU2015150608A
申请日:
2015.11.25
申请国别(地区):
RU
年份:
2017
代理人:
摘要：
FIELD: biotechnology.SUBSTANCE: method for cultivated oat growing under the conditions of soil contamination with oil products involves application of a population of Mg8 (Bacillus sp.) strain. Seed treatment is carried out for 2 hours at a temperature of 18-25°C by a suspension of bacteria with a concentration of 107 microbial cells in 1 ml. A suspension of cultured bacteria transferred from an uncultivated state is obtained as follows: a strain of Mg8 bacteria is accumulated on a dense nutrient medium for 24-48 hours at a temperature of 37°C, then the bacteria are washed with sterile saline, the resulting bacterial suspension is placed into a refrigerator at a temperature of +4 - +5°C for 72 hours for transition of uncultivated bacteria to the cultivated state, a working bacterial concentration of 10microbial cells in1 ml is prepared from the resulting bacterial suspension. Seed are sowed into soil contaminated with up to 10% by oil products, at air temperature of 16-22°C.EFFECT: method allows to grow oat under the conditions of soil contamination with oil and oil products in severe and difficult climatic conditions of the North.3 tbl, 2 ex
