The present invention provides a method for producing a therapeutic agent for liver disease containing adipose tissue-derived stromal cells, which retains excellent therapeutic effects on liver disease of adipose tissue-derived stromal cells, and is also excellent in the convenience of preparation of the therapeutic agent, It is an object of the present invention to provide a therapeutic agent for liver disease obtained by such a production method. The present invention is a therapeutic agent for liver disease comprising adipose tissue-derived stromal cells that have been cryopreserved and thawed at least twice.本発明は、脂肪組織由来間質細胞の肝疾患に対する優れた治療効果を保持しつつ、治療剤の調製の利便性にも優れる、脂肪組織由来間質細胞を含む肝疾患治療剤の製造方法、そのような製造方法により得られる肝疾患治療剤を提供することを課題とする。本発明は、凍結保存および融解を少なくとも２度行った脂肪組織由来間質細胞を含む、肝疾患治療剤である。
