What is described is a method of producing lipid nanoparticles having an average diameter of less than 100 nm, comprising pumping an aqueous buffer solution through a 1st tube by a 1st HPLC pump, pumping an organic lipid solution through a 2nd tube by a 2nd HPLC pump, wherein the 2nd tube intersects the 1st tube perpendicularly within a mixing module, and wherein the organic lipid solution is mixed with the aqueous solution in a turbulent flow within the mixing module.
