PROBLEM TO BE SOLVED: To provide a body fluid separation device that can perform separation easily and quickly, while minimizing an internal inflow of outside air.SOLUTION: A body fluid separation device comprises: a first sealing cap 110; a first cylindrical housing 120 for providing an internal partition wall penetrating through a first connecting hole in the inside of a body; a center cylindrical housing connected with the first cylindrical housing, providing a center partition wall penetrating through a communication hole in the inside of the body, and filled with a body fluid; a second cylindrical housing 140 connected with the center cylindrical housing, and provided with a second connecting hole in the inside of the body; a second sealing cap 150 connected with the second cylindrical housing; and a control unit where a connection end is separably assembled at one end of a fixing hole of a stopper holder part formed on the center partition wall, free edges extending to the sides of first and second connecting holes include first and second stopper bars for opening and closing the first and second connecting holes, respectively, and first and second spaces and an inner space of the center cylindrical housing are connected and disconnected with each other by the first and second stopper bars.SELECTED DRAWING: Figure 1bCOPYRIGHT: (C)2019,JPO&INPIT