A lens module comprises a first lens group, a second lens group and a third lens group, which are arranged from an eye fundus side to an image side in sequence. The first lens group has a positive effective focal length (EFL) and includes a first lens having two convex surfaces respectively facing the eye fundus side and the image side. The second lens group has a positive or negative EFL and includes a plurality of second lenses, wherein the second lens closest to the eye fundus side has a concave surface facing the eye fundus side. The third lens group has a positive EFL and includes a plurality of third lenses, wherein at least one third lens is a cemented lens. The abovementioned lens module decreases the volume of a lens module and reduces the ghosting effect. An eye fundus camera using the abovementioned lens module is also disclosed.