An animal waste collection assembly includes a box that has a first half that is hingedly coupled to the second half. The first half is positioned in an open position and the box may collect animal waste. A pair of wheels is provided and each of the wheels is coupled to the box. Thus, the box may be rolled along a support surface. A handle is coupled to the box and the handle may be manipulated. An actuator is coupled to the handle and the actuator may be manipulated. The actuator is mechanically coupled to the first half such that the actuator selectively urges the first half into the open position wherein the second half is configured to receive animal waste.