The present invention provides a method of producing isothiocyanates, The method comprises forming, in a semisolid or solid callus induction medium, compact callus aggregates from cells obtained from explant material of a plant of a Brassicales plant species. The method comprises transferring cells from the callus aggregates, e.g. transferring the callus aggregates, to a suspension culture in a liquid medium in a shake flask, the liquid medium containing at least one elicitor and/or adsorbent. The method comprises transferring, after culturing in the shake flask, cells from the suspension culture to a further suspension culture in a bioreactor containing the at least one elicitor and/or adsorbent. The method comprises extracting and/or purifying of at least one isothiocyanate from cells obtained from the bioreactor.
