AGRICULTURE AND FOOD DEVELOPMENT AUTHORITY (TEAGASC);UNIVERSITY COLLEGE CORK - NATIONAL UNIVERSITY OF IRELAND, CORK;UNIVERSITY COLLEGE DUBLIN, NATIONAL UNIVERSITY OF IRELAND, DUBLIN
发明人:
BEN LARBI, Nadia,MAROTTA, Mariarosaria,LOSCHER, Christine,HICKEY, Rita Mary,RYAN, Joseph Thomas,PROOS, Sinead
申请号：
EPEP2014/078916
公开号：
WO2015/092047A2
申请日:
2014.12.19
申请国别(地区):
EP
年份:
2015
代理人:
摘要：
This invention provides an oligosaccharide-enriched composition that is derived from a bovine milk whey fraction and comprises at least 23% oligosaccharides (at least 30g per 100g of composition dry weight) having a molecular weight range of 300-1200Da, and at least 20% sialyllactose (at least 20g per 100g of composition dry weight). The oligosaccharide-enriched composition has been found to have a significant immunomodulatory effect (Fig. 1) and has been shown to significantly reduce adhesion of pathogenic bacteria to lung cells and activated pharynx cells, and thereby have a protective effect in humans from respiratory and gastrointestinal infections (Figs 3 and 4). The composition of the invention is derived from a dairy product stream that is substantially free of casein and retains lactose and oligosaccharides, for example demineralised whey powder, demineralised whey, whey permeate, or mother liquor. The dairy product stream is subjected to a micro filtration step to remove whey protein and bacteria, the permeate is then subjected to an ultrafiltration step to enrich for oligosaccharides, and the permeate from the ultrafiltration step is enriched in oligosaccharides having a molecular weight of 300-1200Da, using a chromatographic step.Cette invention concerne une composition enrichie en oligosaccharides qui est dérivée d'une fraction de lactosérum de lait bovin et comprend au moins 23 % d'oligosaccharides (au moins 30 g pour 100 g de poids sec de composition) ayant un poids moléculaire dans la plage de 300-1200 Da et au moins 20 % de sialyllactose (au moins 20 g pour 100 g de poids sec de composition). On a découvert que la composition enrichie en oligosaccharides avait un effet immunomodulateur significatif (Fig. 1) et réduisait significativement l'adhésion de bactéries pathogènes aux cellules pulmonaires et aux cellules de pharynx activés et avaient donc un effet protecteur chez les humains contre les infections respiratoires et gastro-intestinales (Figs 3 e
