Provided is a novel substance that promotes the expression of the filaggrin gene involved in improving the moisture retention function of skin. The filaggrin gene has been discovered to increase and decrease in expression with a rhythmicity of an about 24-hour period. Candidate substances were added； the candidate substances were then screened at the time when expression was at a maximum； and Japanese pepper extract, 3-(1'-piperidine)-propionic acid, geranium oil, cypress oil, rose oil, galbanum oil, pepper oil, basil oil, methyl o-toluate, methyl anthranilate, and dimethyl anthranilate were identified as filaggrin gene expression promoters.本發明提供一種促進與皮膚之保濕功能之提高相關之新穎聚角蛋白微絲基因之表現的物質。本發明發現聚角蛋白微絲基因具有週期約為24小時之節律性而增減其之表現，於添加候補物質後，在表現成為最大之時篩選候補物質，而將山椒萃取物、3-(1'-哌啶)-丙酸、香葉油、柏木油、玫瑰油、格蓬油、胡椒油、羅勒油、鄰甲苯甲酸甲酯、鄰胺基苯甲酸甲酯、鄰甲胺基苯甲酸甲酯鑑定為聚角蛋白微絲基因表現促進劑。
