A puncture apparatus includes a puncture needle that punctures biological tissues a longitudinal-shaped vaginal insertion member that is inserted into the vagina a guiding member an interlock member for interlocking the vaginal insertion member with the guiding member an ultrasonic probe that is provided in a distal portion of the vaginal insertion member to transmit and receive ultrasonic waves and an ultrasonic marker that is provided in the guiding member to be able to recognize an ultrasonic image which is obtained from the ultrasonic waves transmitted and received from the ultrasonic probe. The interlock member has a through-hole into which the puncture needle is inserted, and the through-hole is a supporting portion that movably supports the puncture needle to guide the distal end of the puncture needle to the ultrasonic marker.
