The disclosed is a kind of multiple modified derivatives of gelatin having not only the structure of formula (I) but also one of structures of formula (II), (III), and (IV) as well. Wherein, G refers to gelatin residue comprising type A, type B and the type of gene recombination R1 refers to allylene, or a linkage group with amide R2 refers to alkyl, or aryl R3 refers to alkylene R4 refers to carboxyl or carboxylate. The multiple modifying ways of gelatin comprise the hydrophobic modification on the amino group of gelatin through amide bond, carboxylation on the amino group of gelatin through amide bond, thiolation on the carboxyl group of gelatin, and thiolation following carboxylation on amino group of gelatin through amide bond. In addition, the invention also discloses the crosslinked material made of multiple modified derivatives of gelatin. The multiple modified derivatives of gelatin have flexible chemical structures and many properties. Their crosslinked gelatin material can be used the matrix for cell growth etc.
