Embodiments herein report compositions, methods and uses for dengue-4 (DEN-4) virus constructs. Some embodiments concern a composition that includes, but is not limited to, DEN-4 virus constructs disclosed herein that alone or in combination with other constructs can be used in a vaccine composition to induce an immune response in a subject. In certain embodiments, compositions can include constructs of more than one serotypes of dengue virus, such as dengue-1 (DEN-1) virus, dengue-2 (DEN-2) virus, or dengue-3 (DEN-3) virus in combination with DEN-4 virus constructs disclosed herein. In other embodiments, DEN-4 constructs disclosed herein can be combined in a composition with other flavivirus constructs to generate a vaccine against more than one flavivirus. Other embodiments provide methods and uses for DEN-4 virus constructs in vaccine compositions that when administered to a subject induce an immune response in the subject against DEN-4 that is improved by modified constructs compared to other vaccine compositions.在此之具體例記述登革熱4型(DEN-4)病毒建構物的組成物、方法及用途。一些具體例有關一種組成物,其包括,但不限於,在此所揭示之DEN-4病毒建構物,單獨或結合其它建構物,可用在疫苗組成物中,用以在個體中誘發免疫反應。在某些具體例中,組成物可包括超過一種登革熱病毒血清型之建構物,諸如登革熱1型(DEN-1)病毒、登革熱2型(DEN-2)病毒或登革熱3型(DEN-3)病毒,結合在此所揭示之DEN-4病毒建構物。在其它具體例中,在此所揭示之DEN-4建構物可於組成物中結合其它黃病毒建構物,產生抗超過一種黃病毒之疫苗。其它具體例提供DEN-4病毒建構物於疫苗組成物中之方法以及用途,其當投與至個體時,會在該個體中誘發抗DEN-4之免疫反應,其與其它疫苗組成物相比,已經過建構物修飾之改良。
