Gene therapeutic DNA vector based on VTvaf17 gene therapeutic DNA vector, carrying the target gene selected from the group of genes KRT5, KRT14, LAMB3, COL7A1 to increase the expression level of these target genes, method for its preparation and use, strain Escherichia coli SCS110-AF / VTvaf17- KRT5 or Escherichia coli SCS110-AF / VTvaf17-KRT14 or Escherichia coli SCS110-AF / VTvaf17-LAMB3 or Escherichia coli SCS110-AF / VTvaf17-COL7A1 carrying a gene therapy DNA vector, a method for its production, a method for the industrial production of gene therapy DNA of vector
The invention refers to genetic engineering and can be used in biotechnology, medicine, and agriculture for the manufacture of gene therapy products. Gene therapy DNA vector based on the gene therapy DNA vector VTvaflV carrying the therapeutic gene selected from the group of KRT5, KRT14, LAMB 3, and COL7A1 genes was constructed in order to increase the expression level of this therapeutic gene in humans and animals, while gene therapy DNA vector VTvafl7-KRT5, or VTvafl7-KRTl4, or VTvafl7-LAMB3, or VTvafl7-COL7Al has the nucleotide sequence SEQ ID No. 1, or SEQ ID No. 2, or SEQ ID No. 3, or SEQ ID No. 4, respectively. The gene therapy DNA vector contains no nucleotide sequences of viral origin and no antibiotic resistance genes, which ensures its safe use for gene therapy in humans and animals. A method of obtaining the specified vector, the use of the vector, a strain of Escherichia coli carrying the specified vector, and a method of industrial production of the specified vector are also provided.
