GENE-THERAPEUTIC DNA-VECTOR BASED ON THE GENE-THERAPEUTIC DNA-VECTOR VTvaf17, CARRYING THE TARGET GENE SELECTED FROM THE GROUP OF GENES COL1A1, COL1A2, BMP2, BMP7, TO INCREASE THE LEVEL OF EXPRESSION OF THESE TARGET GENES, METHOD FOR PRODUCTION AND USE THEREOF, ESCHERICHIA COLI SCS110-AF/VTvaf17-COL1A1 STRAIN OR ESCHERICHIA COLI SCS110-AF/VTvaf17-COL1A2 OR ESCHERICHIA COLI SCS110-AF/VTvaf17-BMP2 OR ESCHERICHIA COLI SCS110-AF/VTvaf17-BMP7, CARRYING A GENE-THERAPEUTIC DNA VECTOR, METHOD FOR PRODUCTION THEREOF, A METHOD FOR INDUSTRIAL PRODUCTION OF A GENE-THERAPEUTIC DNA-VECTOR
Savelieva Natalia (AT),Савелиева Наталиа (AT),Lazarev Vasilij Nikolaevich (RU),Лазарев Василий Николаевич (RU),Shmarina Galina Vasilevna (RU),Шмарина Галина Васильевна (RU)
申请号：
RU2018131708
公开号：
RU0002707537C1
申请日:
2018.09.04
申请国别(地区):
RU
年份:
2019
代理人:
摘要：
FIELD: genetics.SUBSTANCE: invention relates to genetic engineering. Described is a gene-therapeutic DNA-vector based on a gene-therapeutic DNA-vector VTvaf17, carrying a target gene selected from a group of genes COL1A1, COL1A2, BMP2, BMP7, to increase the level of expression of this target gene in the human body and animals, wherein the gene-therapeutic DNA vector VTvaf17-COL1A1, or VTvaf17-COL1A2, or VTvaf17-BMP2, or VTvaf17-BMP7 has the nucleotide sequence SEQ ID No. 1 or SEQ ID No. 2 or SEQ ID No. 3 or SEQ ID No. 4, respectively. Each of the created genotyping DNA-vectors: VTvaf17-COL1A1, or VTvaf17-COL1A2, or VTvaf17-BMP2, or VTvaf17-BMP7 due to the VTvaf17 vector part limited size, which does not exceed 3,200 base pairs, is capable of efficiently penetrate into cells and to express the target gene cloned therein, selected from the group of COL1A1, COL1A2, BMP2, BMP7 genes. Gene-therapeutic DNA-vector does not contain nucleotide sequences of viral origin and there are no antibiotic resistance genes, providing the possibility of its safe application for genetic therapy of humans and animals. There is also developed a method for obtaining a gene-therapeutic DNA-vector on the basis of gene-therapeutic DNA-vector VTvaf17 carrying the target gene selected from a group of genes: COL1A1, COL1A2, BMP2, BMP7, which consists in that each gene-therapeutic DNA vector: VTvaf17-COL1A1, or VTvaf17-COL1A2, or VTvaf17-BMP2, or VTvaf17-BMP7, are obtained as follows: coding part of the target gene from the group of COL1A1, COL1A2, BMP2, BMP7 genes is cloned into a VTvaf17 DNA-vector and a gene-therapeutic DNA-vector VTvaf17-COL1A1, SEQ ID No. 1 or VTvaf17-COL1A2, SEQ ID No. 2 or VTvaf17-BMP2, SEQ ID No. 3, or VTvaf17-BMP7, SEQ ID No. 4 is obtained, respectively. Method for using the created DNA-vector based on the gene-therapeutic VTvaf17 DNA-vector carrying a target gene selected from a group of genes: COL1A1, COL1A2, BMP2, BMP7, to increase expression level of these target gen
