The endoscope diagnostic apparatus includes a light source emitting white light and two or more kinds of excited light with different center wavelengths for emitting two or more kinds of self-fluorescence from a self-fluorescent material, an imaging unit which receives reflected light of white light to image a normal light image, and receives self-fluorescence emitted from the self-fluorescent material to image self-fluorescent images, and a light source control unit which has compensation coefficients for preventing emission intensity from being lowered depending on the volume of blood when the two or more kinds of excited light are absorbed by blood, and compensates the ratios of emission intensity of the two or more kinds of excited light using the calculated compensation coefficients so as to exclude the influence of absorption of light by blood in each of the two or more kinds of excited light.