A sealed sterile device for the aseptic separation and concentration of biological components, which includes: a tube sealed at first end and which has a mouth at second end； a plunger of flexible material, without an actuation rod, installed inside the tube with the possibility of longitudinal movement, and which has a hole for charging with a fluid to be separated and concentrated and discharging the separated fractions, the hole being provide with a valved female Luer lock connection, the plunger also having a purging duct provided with a self-sealing filter for the automatic purging of the air/gas contained in the tube during the filling of the tube with the fluid to be separated and concentrated； and a cap for protecting the connection-plunger assembly, couplable to the mouth of the tube.
