The invention relates to a sealed sterile device for the aseptic separation and concentration of biological components, which comprises at least: a tube (1) sealed at a first end (11) and which comprises a mouth (12) at a second end； a plunger (2) of flexible material, without an actuation rod, installed inside the tube (1) with the possibility of longitudinal movement, and which comprises a hole (21) for charging with a fluid to be separated and concentrated and discharging the separated fractions, the hole (21) being provided with a valved female Luer lock connection (22), the plunger also comprising a purging duct (23) provided with a self-sealing filter (24) for the automatic purging of the air/gas contained in the tube (1) during the filling of the tube (1) with the fluid to be separated and concentrated； and a cap (3) for protecting the connectionplunger assembly, couplable to the mouth of the tube.
