The Board of Trustees of the University of Illinois
发明人:
Smith Sheena N.,Kranz David M.
申请号：
US201815952942
公开号：
US2018305434(A1)
申请日:
2018.04.13
申请国别(地区):
美国
年份:
2018
代理人:
摘要：
The use of model T cell receptors (TCRs) as scaffolds for in vitro engineering of novel specificities is provided. TCRs with de novo binding to a specific peptide-major histocompatibility complex (MHC) product can be isolated by: 1) mutagenizing a T cell receptor protein coding sequence to generate a variegated population of mutants (a library), 2) selection of the library of TCR mutants with the specific peptide-MHC, using a process of directed evolution and a “display” methodology (e.g., yeast, phage, mammalian cell) and the peptide-MHC ligand. The process can be repeated to identify TCR variants with improved affinity for the selecting peptide-MHC ligand.
