A docking station for maintaining sterilization of temporarily disconnected male medical line connectors. The docking station has a plurality of docking bays, each of which retain an ampule therein containing a sterilization fluid and has an access mouth exteriorly exposed when the ampule is secured in its respective bay. The mouth of the ampule is provided with a female medical line connection for temporarily receiving and securing one of the male medical line connector temporarily disconnected from a patient. A tubular tip and a concentric connection collar of the one of the male medical line connector is thereby immersed in the sterilization fluid for sterilization while docked. Each of the ampules is secured against rotation in a clockwise direction in their respective docking bay to facilitate threadable connection of one of the male medical line connector. However, the ampules are permitted to freely rotate in the counterclockwise direction in their respective bays to prevent disconnection of one of the male medical line connectors therefrom while the connected ampules are retained in their respective bays. This prevents accidental reuse of an ampule. The ampules are provided with laterally extending wings which are received in recesses in their respective bays. The recesses have inclined ramp surfaces for engaging the bottom of the wings and thereby ejecting the ampules out of the bays when rotated in the counterclockwise direction.
