Disinfection of rooms by germicidal ultraviolet characterized by having means for the filtration and retention of particles above 0.1 microns, formed by an inlet grille at the inlet mouth (2), with a primary pre-filtration stage formed by a pre-filter F5 or F7 (3), a filtration stage formed by a filter type F9 (4), to warn of the saturation of the filters, has at least one pressure switch (15), an extraction system using a turbine formed by fan (5) that is soundproofed inside an envelope covered with sound-absorbing acoustic insulation (6) and where uniformly distributed mounted emitters interrupt the passage of air, forcing it to be in close contact with the surface of at least one germicidal lamp (8) with an intensity at 1 meter minimum of 9 μwcm2, preferably from 9 to 36 μwcm2 and a main wavelength of 254 nm ozone-free; the disinfection unit allows both disinfection of ventilation and air conditioning systems by having inlet (2) and outlet (10) ports for connection to the installation, as well as means to directly irradiate on the surfaces of a room by having at least one manhole cover (11) whose opening allows the radiation from the germicidal lamps to hit the facing surfaces; the assembly can be movable presenting wheels (12) for its transport and a guide handle. (Machine-translation by Google Translate, not legally binding)