It is intended to provide a separation container by which contamination with an unnecessary component can be prevented. A separation container (1) for collecting and separating a subject to be separated from a sample to the bottom thereof by centrifuging which has a container body (11) and compartments, characterized in that the above-described container body (11) is a tubular container being long along an axial direction with an opening at the upper end and the bottom at the lower end, the above-described compartments have a partition (12) formed along the above-described axial direction of the container body as described above, the inner space of the above-described container body (11) is divided into a sample lead-in chamber (14) and a separation subject lead-out chamber (15) by the above-described partition (12), the sample lead-in chamber (14) and the separation subject lead-out chamber (15) as described above are connected to each other via a through hole (13) formed in the lower part of the partition (12), a collection tube can be inserted via the above-described opening into the lower part of the separation subject lead-out chamber (15) as described above, and the above-described separation subject located in the lower part of the above-described separation subject lead-out chamber (15) can be collected by the above-described collection tube and harvested outside the container body (11) as described above.On vise à proposer un conteneur de séparation par lequel une contamination par un composant non nécessaire peut être empêchée. Linvention porte sur un conteneur de séparation (1) pour collecter et séparer un objet devant être séparé dun échantillon à la partie inférieure de celui-ci par centrifugation qui a un corps de conteneur (11) et des compartiments, caractérisé par le fait que le corps de conteneur (11) décrit ci-dessus est un conteneur tubulaire qui est allongé suivant une direction axiale avec une ouverture à lextrémité supérieure et le fond à lext